Dojindo Cell Counting Kit-8 CCK-8细胞计数试剂盒检测方法简介

CCK-8 Kit 细胞活性检测试剂中含有WST-8，化学名：2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基） -5-（2,4-二磺酸苯）-2H-四唑单钠盐]，它在电子载体1-甲氧基-5-甲基吩嗪鎓*（1-Methoxy PMS）的作用下被细胞线粒体中的脱氢酶还原为具有高度 水溶性的黄色甲瓒产物（Formazan）。生成的甲瓒物的数量与活细胞的数量成正比。用酶联免疫检测仪在450nm波长处测定其光吸收值，可间接反映活 细胞数量。该CCK-8 Kit方法已被广泛用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞增殖试验、细胞毒性试验以及药敏试验等。
 

Dojindo Cell Counting Kit-8 CCK-8细胞计数试剂盒的优点：

● CCK-8 Kit试剂盒使用方便，省去了洗涤细胞，不需要放射性同位素和有机溶剂；
● CCK-8法能快速检测；
● CCK-8法的检测灵敏度很高，甚至可以测定较低细胞密度；
● CCK-8法的重复性优于MTT法；
● CCK-8法对细胞毒性小；
● CCK-8 Kit细胞活性检测试剂中为1瓶溶液，毋需预制，即开即用。
 

的缺点：

● 与MTT法相比，CCK8和XTT的价格比较贵；
● CCK8试剂的颜色为淡红色，与含酚红的培养基颜色接近，不注意的话容易产生漏加或多加。
 

 主要产品特性及应用

★ 操作简便，省时省力
★ 水溶性，不需要换液，尤其适合于悬浮细胞
★ 无需放射性同位素和有机溶剂，对细胞毒性低
★ 为1瓶溶液，毋需预制，即开即用
★ 适合于高通量药物筛选
 

 的用途：

药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验

Storage Condition : 0-5^oC
Shipping Condition : ambient temperature

Product Description
Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, WST-8*, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1.
*Patent No. US6,063,587 & CA2,251,850




Cell viability detection mechanism with CCK-8

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Procedure
Cell Counting Kit-8 requires 3 simple steps.


The handling time of Cell Counting Kit-8 is the shortest among it’s competitors. Only 15 minutes of handling time for Cell Counting Kit-8 where as longer handling time is required for both MTS and MTT assay.

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Stability
Cell Counting Kit-8 is a ready-to-use solution. It is also stabe at 4ºC for 1 year. Your assay can be done anytime without thaw and freeze.

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Sensitivity
CCK-8 (WST-8) is the highest sensitive dye for the cell based assay.

Comparison of the sensitivity using tetrazolium dye

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Cytotoxicity of Reagents
Only in CCK-8, continious culture is possible without killing cells.

Observation of Cytotoxicity after incubation with each reagent.
HeLa cells were incubated with WST-8 (CCK-8), WST-1, or MTS at 24 hours from addition of those reagents. 

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